T7EI

T7EI (T7 Endonuclease I)
BBF10K_003319
source
Escherichia phage T7 (Bacteriophage T7)

DNA Endonuclease; catalyzes the cleavage of DNA mismatches and non-β DNA structures including Holliday junctions and cruciform leaving 3'-OH and 5'-phosphate. best at C mismatches and does not recognize all DNA mismatches. To a lesser extent cleaves across a nick in dsDNA; Key enzyme for use in genome editing mutation detection workflows

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T7EI
plasmid size
2573
insert size
471
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.