T4 PGD

T4 PGD (T4 Endonuclease V)
BBF10K_003320
source
Enterobacteria phage T4 (bacteriophage T4)

T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. The N-glycosylase activity releases cis-syn cyclobutane pyrimidine dimers, including T^T, T^C and C^C, generating an AP site. The AP lyase activity cleaves an AP site via β-elimination, creating a 1 nucleotide DNA gap with 3'-α, β-unsaturated aldehyde and 5'-phosphate termini. Also known as T4 Endonuclease V, this enzyme in combination with other enzymes from E. coli (UDG, fpg, Endonuclease IV, Endonuclease VIII), Bst-DNA pol and Taq/Tth-DNA Ligase forms the proprietary NEB Enzyme mix preCR

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Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T4 PGD
plasmid size
2540
insert size
438
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.