AB_pTac

AB_pTac
BBF10K_003380
source
N/A

The tac promoter (a strong E.coli promoter that does not require any special RNA polymerases) defined as an AB part

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - GGAG … TACT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-AB_pTac
plasmid size
2415
insert size
75
origin
ColE1
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.