BT1_BCD12

BT1_BCD12
BBF10K_003387
source
N/A

A strong BiCistronic Designed (BCDs) RBS. BCDs reduce the variation in RBS-CDS pairing. Defined as a BN1 part.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - TACT … CCAT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-BT1_BCD12
plasmid size
2413
insert size
93
origin
ColE1
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.