T4T5_Int_ThiolS

T4T5_Int_ThiolS
BBF10K_003461
source
N/A

Modified intein, cleavable by addition of thiol-containing molecules such as cysteine, DTT, and beta-mercaptoethanol. Defined as a T4T5 part.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AGGT … CGCT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T4T5_Int_ThiolS
plasmid size
2720
insert size
600
origin
ColE1
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.