T4T5_Int_SaltS

T4T5_Int_SaltS
BBF10K_003462
source
N/A

Salt-cleavable intein. Cleaves itself in the presence of 1.5 M NaCl (has little to no activity at 1 M NaCl). Usable as self-cleaving c-terminal tag. Defined as a T4T5 part.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - TTCG … CGGC - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T4T5_Int_SaltS
plasmid size
2735
insert size
615
origin
ColE1
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.