HIV-RT

His-tagged HIV-RT p66
BBF10K_003494
source
Human immunodeficiency virus 1 (HIV-1)

Human Immunodeficiency Virus reverse transcriptase expression plasmid. Apparently expressed better than most other RTs in E.coli. Originally created by the Dickinson Lab at the University of Chicago

attr.
Dickinson lab, UC Chicago

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin

expression

strain
Escherichia coli
promoter
T7
inducer
LacI

cloning

method
N/A
enzyme
N/A
overhangs
3' - N/A … N/A - 5'

sequencing

forward primer
N/A
reverse primer
N/A

Construct

plasmid name
pET30A-HIV-RT
plasmid size
6930
insert size
1686
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

N/A

intellectual property

We are unaware of third-party property rights claims on uses of this item.