Bst-LF

Bst-LF DNA polymerase
BBF10K_003496
source
Bacillus stearothermophilus

Bst-LF expression plasmid. Used in Loop-Mediated Isothermal Amplification (LAMP). Originall created by the Open Bioeconomy Lab

attr.
Alex Brown, Nat'l Jewish Health

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
Escherichia coli
promoter
T7
inducer
LacI

cloning

method
N/A
enzyme
N/A
overhangs
3' - N/A … N/A - 5'

sequencing

forward primer
N/A
reverse primer
N/A

Construct

plasmid name
pET24a-Bst-LF
plasmid size
7476
insert size
1776
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

N/A

intellectual property

We are unaware of third-party property rights claims on uses of this item.