pBuild

pOpen_v3
BBF10K_003498
source
Synthetic

Robust, flexible E. coli cloning vector. Designed to be compatible with multiple cloning strategies including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard.

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
Escherichia coli
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BbsI, BtgZI, AarI
overhangs
3' - varies … varies - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen_build
plasmid size
2687
insert size
690
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

citations

N/A

intellectual property

We are unaware of third-party property rights claims on uses of this item.