EcPPA Pyrophosphatase

Inorganic pyrophosphatase from E.coli
BBF10K_003504
source
Escherichia coli

Used in NASBA test. Used for generation of phosphate for in-vitro reactions. This is an expression vector, inducible with IPTG and his-tagged

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
Escherichia coli
promoter
T7
inducer
LacIq

cloning

method
N/A
enzyme
N/A
overhangs
3' - N/A … N/A - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-EcPPA Pyrophosphatase
plasmid size
4404
insert size
2403
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

No Value

intellectual property

We are unaware of third-party property rights claims on uses of this item.