eGFP

eGFP coded for mammalian expression
BBF10K_003539
source
SARS-CoV-2

Lentiviral expression of eGFP; transient expression and generate lentivirus

attr.
Roth Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
30 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
Homo sapiens
promoter
N/A
inducer
N/A

cloning

method
Gateway
enzyme
N/A
overhangs
3' - N/A … N/A - 5'

sequencing

forward primer
N/A
reverse primer
N/A

Construct

plasmid name
peGFP
plasmid size
9642
insert size
840
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

N/A

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.