MMSYN1_0524
Ribosomal RNA small subunit methyltransferase HBBF10K_000248
source
Synthetic bacterium JCVI-Syn3.0
Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.
attr.
Anton Jackson-Smith and Bioe80 class
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-MMSYN1_0524
plasmid size
2984
insert size
927
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE