pBad-Xis/Int Reset Flipper
pBad-Xis/Int Reset FlipperBBF10K_000494
source
No Value
Used to switch DNA registers from LR to BP state. Expresses Bxb1 integrase and excisionase under the control of the pBAD promoter inducible by Arabinose. Regulated ColE1 ori (50-75 copies); Chloramphenicol resistance
attr.
Drew Endy
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Chloramphenicol
expression
strain
No Value
promoter
pBAD
inducer
Arabinose
cloning
method
No Value
enzyme
No Value
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
pBAD-F
reverse primer
No Value
Construct
plasmid name
pBad-Xis/Int Reset Flipper
plasmid size
5738
insert size
No Value
origin
ColE1
copy number
50-75
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere