RAD Module G8-C1
RAD Module G8-C1BBF10K_000495
source
No Value
Used to switch DNA registers from BP to LR state and back from LR to BP state using two different inducers (SR-latch). Expresses Bxb1 integrase only under the control of the pTET promoter inducible by Anhydrotetracycline (AtC). Expresses both Bxb1 integrase and excisionase under the control of the pBAD promoter inducible by Arabinose. Regulated ColE1 ori (50-75 copies); Chloramphenicol resistance.
attr.
Drew Endy
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Chloramphenicol
expression
strain
No Value
promoter
pTET promoter, pBAD promoter
inducer
Anhydrotetracycline (AtC), Arabinose
cloning
method
No Value
enzyme
No Value
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
CAT-R
reverse primer
No Value
Construct
plasmid name
RAD Module G8-C1
plasmid size
7485
insert size
No Value
origin
ColE1
copy number
50-75
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere