OR gate
OR gateBBF10K_000498
source
No Value
Two-input recombinase logic OR gate. Produces GFP when one or two recombinases have been expressed and acted on the DNA registers (binary output number: 0111). Consists of a promoter followed by one transcriptional terminator flanked by nested parallel recombination sites. Recombination excises the terminator and allows for the expression of downstream GFP. Operated by recombinases Bxb1 and TP901 (e.g. using the dual controller). Low copy pSC101 ori (5-10 copies)- Ampicilin resistance.
attr.
Drew Endy
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
N/A
enzyme
N/A
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
No Value
reverse primer
No Value
Construct
plasmid name
OR gate
plasmid size
4800
insert size
No Value
origin
pSC101
copy number
5-10
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere