Dual-recombinase-controller
Dual-recombinase-controllerBBF10K_000501
source
No Value
Contains pTET-Bxb1 integrase inducible by anhydrotetracyclin (AtC) and pBAD-TP901 integrase inducible by arabinose. Can be used to control two-input logic gates. Regulated ColE1 ori (50-75 copies); Chloramphenicol resistance.
attr.
Drew Endy
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Chloramphenicol
expression
strain
No Value
promoter
pTET promoter, pBAD promoter
inducer
Anhydrotetracycline (AtC), Arabinose
cloning
method
N/A
enzyme
N/A
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
No Value
reverse primer
No Value
Construct
plasmid name
Dual-recombinase-controller
plasmid size
6614
insert size
No Value
origin
pUC
copy number
50-75
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere