yD1E

S. cerevisiae codon optimized Dengue serotype 1 Envelope glycoprotein
BBF10K_000590
source
Dengue virus 1

Binds to host cell surface receptor and mediates fusion between viral and cellular membranes

attr.
Nguyen Ngoc Luong

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin

expression

strain
Saccharomyces cerevisiae 2805
promoter
Gal1/TDH3-1
inducer
Galactose/No inducer

cloning

method
Restriction digestion
enzyme
BamHI/SalI
overhangs
3' - GGATCC … GTCGAC - 5'

sequencing

forward primer
No Value
reverse primer
No Value

Construct

plasmid name
pOPEN00231
plasmid size
No Value
insert size
1485
origin
E. coli OriC
copy number
500-700

Safety

BSL
1

other information

N/A

References

primary references

NCBI
UniProt
Available Elsewhere
No Value

protocols and instructions

No Value

citations

No Value

intellectual property

We are unaware of third-party property rights claims on uses of this item as of we.