cysS
cysteine--tRNA ligaseBBF10K_000772
source
Bacillus subtilis 168
catalyzes a two-step reaction; charges a cysteineby linking its carboxyl group to the alpha-phosphate ofATP then transfers the aminoacyl-adenylate to its tRNA
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-cysS
plasmid size
3421
insert size
1401
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE