secA
protein translocase subunit SecABBF10K_001010
source
Bacillus subtilis 168
functions in protein export; can interact withacidic membrane phospholipids and the SecYEG proteincomplex; binds to preproteins; binds to ATP and undergoesa conformational change to promote membrane insertion ofSecA/bound preprotein; ATP hydrolysis appears to driverelease of the preprotein from SecA and deinsertion ofSecA from the membrane; additional proteins SecD/F/YajCaid SecA recycling; exists in an equilibrium betweenmonomers and dimers; may possibly form higher orderoligomers; proteins in this cluster correspond SecA1;SecA2 is not essential and seems to play a role insecretion of a subset of proteins
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-secA
plasmid size
4549
insert size
2526
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE