era
GTP-binding protein EraBBF10K_001499
source
Mesoplasma florum L1
Era; Escherichia coli Ras-like protein; Bex;Bacillus Era-complementing segment; essential protein inEscherichia coli that is involved in many cellularprocesses; GTPase; binds the cell membrane throughapparent C-terminal domain; mutants are arrested duringthe cell cycle; Streptococcus pneumoniae Era binds to RNAand Escherichia coli Era binds 16S rRNA and 30S ribosome
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-era
plasmid size
2969
insert size
906
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE