Mfl278
Guanosine-3', 5'-bis(Diphosphate)3'-pyrophosphohydrolaseBBF10K_001511
source
Mesoplasma florum L1
In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-Mfl278
plasmid size
4361
insert size
2298
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE