infB
translation initiation factor IF-2BBF10K_001528
source
Mesoplasma florum L1
Protects formylmethionyl-tRNA from spontaneoushydrolysis and promotes its binding to the 30S ribosomalsubunits during initiation of protein synthesis. Alsoinvolved in the hydrolysis of GTP during the formation ofthe 70S ribosomal complex
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-infB
plasmid size
3953
insert size
1890
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE