secY
preprotein translocase subunit SecYBBF10K_002604
source
Mycoplasma pneumoniae M129
forms heterotrimeric complex in the membrane; inbacteria the complex consists of SecY which forms thechannel pore and SecE and SecG; the SecG subunit is notessential; in bacteria translocation is driven via theSecA ATPase
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-secY
plasmid size
3496
insert size
1434
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE