fpg
formamidopyrimidine-DNA glycosylaseBBF10K_002805
source
Mycoplasma pneumoniae M129
Involved in base excision repair of DNA damaged byoxidation or by mutagenic agents. Acts as DNA glycosylasethat recognizes and removes damaged bases
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-fpg
plasmid size
2896
insert size
834
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE