K12polLF (exo)
DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-)BBF10K_003249
source
Escherichia coli (strain K12)
DNA polymerase fragment used in flourescent labelling for microarray, dA and dT tailling, and important in the process of ligating DNA adapters to DNA fragments. Frequently used in prepararing DNA libraries for Next-Gen sequencing. The exo-Klenow polymerase is a mutant polymerase from the Klenow DNA polymerase large fragment that lacks 5'-3' polymerase activity and 3'-5' exonuclease activity."
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-K12polLF (exo)
plasmid size
3353
insert size
1230
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE