ECOpolA
E. coli DNA Polymerase IBBF10K_003250
source
Escherichia coli (strain K12)
DNA polymerase that (in addition to polymerase activity) exhibits 3'-5' and 5'-3' exonuclease activity. Able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-ECOpolA
plasmid size
4910
insert size
2787
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE