phi29pol

phi29 DNA Polymerase
BBF10K_003264

source

Bacillus phage phi29

DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

attr.

Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain

{shipping_strain}

growth conditions

37 C, shaking 300 rpm

antibiotic

ampicillin

expression

strain

N/A

promoter

N/A

inducer

N/A

cloning

method

GoldenGate

enzyme

BsaI

overhangs

3' - AATG … GCTT - 5'

sequencing

forward primer

M13 For

reverse primer

M13 Rev

Construct

plasmid name

pOpen-phi29pol

plasmid size

3851

insert size

1728

origin

ColE1 High Copy

copy number

high (3-500)

genbank file

Safety

BSL

BSL1

other information

No Value

References

primary references

NCBI

No Value

UniProt

P03680

Available Elsewhere

FALSE

protocols and instructions

https://docs.google.com/document/d/1c8PyT1Z9cWYUif4XQzcWl8MPbqVuL8-RNKCAancaVQU/edit, https://openbioeconomy.org/projects/expression-guide/

citations

Nucleotide sequence of the late region of Bacillus phage phi 29 completes the 19,285-bp sequence of phi 29 genome. Comparison with the homologous sequence of phage PZA https://pubmed.ncbi.nlm.nih.gov/3803926/

intellectual property

We are unaware of third-party property rights claims on uses of this item.