9N7lig
9 Degrees North DNA LigaseBBF10K_003274
source
Thermococcus sp. 9°N-7
9°N DNA Ligase active at elevated temperatures (45°C-90°C). Depends on the use of ATP and not NAD+ as a cofactor.
attr.
Chiara Gandini, Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-9N7lig
plasmid size
3803
insert size
1680
origin
ColE1 High Copy
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE