Pfulig

Pfu DNA Ligase
BBF10K_003275

source

Pyrococcus furiosus

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Catalyzes template-dependent ligation at temperatures of about 30° C. to about 80° C., and substantially retains its catalytic activity when subjected to temperatures of about 85° C. to about 100° C.

attr.

Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain

{shipping_strain}

growth conditions

37 C, shaking 300 rpm

antibiotic

ampicillin

expression

strain

N/A

promoter

N/A

inducer

N/A

cloning

method

GoldenGate

enzyme

BsaI

overhangs

3' - AATG … GCTT - 5'

sequencing

forward primer

M13 For

reverse primer

M13 Rev

Construct

plasmid name

pOpen-Pfulig

plasmid size

3809

insert size

1686

origin

ColE1 High Copy

copy number

high (3-500)

genbank file

Safety

BSL

BSL1

other information

No Value

References

primary references

NCBI

No Value

UniProt

P56709

Available Elsewhere

FALSE

protocols and instructions

https://docs.google.com/document/d/1c8PyT1Z9cWYUif4XQzcWl8MPbqVuL8-RNKCAancaVQU/edit, https://openbioeconomy.org/projects/expression-guide/

citations

Sillero, M. A. G., Montes, M., de Diego, A., del Valle, M., Atencia, E., & Sillero, A. (2002). Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di) nucleoside polyphosphates. Extremophiles, 6(1), 45-50.

intellectual property

We are unaware of third-party property rights claims on uses of this item.