ALPI

CIP (calf intestinal phosphatase)
BBF10K_003278
source
Bos taurus (bovine)

CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-ALPI
plasmid size
3725
insert size
1602
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.