MMLV_RT (mut H)

Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
BBF10K_003285

source

Moloney Murine Leukemia Virus

Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

attr.

Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain

{shipping_strain}

growth conditions

37 C, shaking 300 rpm

antibiotic

ampicillin

expression

strain

N/A

promoter

N/A

inducer

N/A

cloning

method

GoldenGate

enzyme

BsaI

overhangs

3' - AATG … GCTT - 5'

sequencing

forward primer

M13 For

reverse primer

M13 Rev

Construct

plasmid name

pOpen-MMLV_RT (mut H)

plasmid size

4109

insert size

1986

origin

ColE1 High Copy

copy number

high (3-500)

genbank file

Safety

BSL

BSL1

other information

No Value

References

primary references

NCBI

No Value

UniProt

No Value

Available Elsewhere

FALSE

protocols and instructions

https://docs.google.com/document/d/1c8PyT1Z9cWYUif4XQzcWl8MPbqVuL8-RNKCAancaVQU/edit, https://openbioeconomy.org/projects/expression-guide/

citations

Kotewicz, M.L., D'Alessio, J.M., Driftmier, K.M., Blodgett, K.P. and Gerard, G.F., 1985. Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli. Gene, 35(3), pp.249-258. This variant is published in: Liu, S., Goff, S. P., & Gao, G. (2006). Gln84 of moloney murine leukemia virus reverse transcriptase regulates the incorporation rates of ribonucleotides and deoxyribonucleotides. FEBS letters, 580(5), 1497-1501.

intellectual property

We are unaware of third-party property rights claims on uses of this item.