T4 PNK
T4 Polynucleotide KinaseBBF10K_003317
source
Enterobacteria phage T4 (bacteriophage T4)
The most commonly used polynucleotide kinase. Used for: 5' phosphorylation of DNA/RNA for subsequent ligation; end labeling DNA or RNA for probes and DNA sequencing; removal of 3' phosphoryl groups
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-T4 PNK
plasmid size
3029
insert size
927
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE