DNA Topoisomerase 1B Vaccinia Virus

DNA Topoisomerase 1B Vaccinia Virus
BBF10K_003324
source
No Value

Used in TOPO cloning. Recognizes the DNA sequence 5´-(C/T)CCTT-3' and digests double stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the DNA and the topoisomerase. If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands and releasing topoisomerase.

attr.
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Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-DNA Topoisomerase 1B Vaccinia Virus
plasmid size
3068
insert size
966
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

https://www.ncbi.nlm.nih.gov/pubmed/2541548 (1989), first partial isolation here https://www.ncbi.nlm.nih.gov/pubmed/17115 (1977)

intellectual property

We are unaware of third-party property rights claims on uses of this item.