Exonuclease III E. coli
Exonuclease III E. coliBBF10K_003330
source
Escherichia coli
Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA. Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 3' to 5' direction. Ideal for site-directed mutagenesis, preparation of single-stranded DNA for dideoxy sequencing, preparation of nested deletions in double-stranded DNA
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-Exonuclease III E. coli
plasmid size
2930
insert size
807
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE