Firefly Luciferase [Photinus pyralis] (Fluc)
Firefly Luciferase [Photinus pyralis] (Fluc)BBF10K_003368
source
Photinus pyralis
luciferin 4-monooxygenase from common eastern firefly. Produces green light with a wavelength of 562 nm. Most commonly used luciferase reporter.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-Firefly Luciferase [Photinus pyralis] (Fluc)
plasmid size
3776
insert size
1653
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE