Luciferase [Luciola cruciata] (Japanese firefly)
Luciferase [Luciola cruciata] (Japanese firefly)BBF10K_003369
source
Gaussia princeps/Luciola cruciata?
Luciferase from Gaussia princeps. At 19kD, it is the smallest known luciferase and its catalytic properties make it one of the “brightest” known. Additionally, multiple disulfide bonds contribute to the protein stability at elevated temperatures. The first report of in vivo imaging using Gluc demonstrated sensitivity that is several orders of magnitude higher than the first generation luciferases, Firefly (Fluc) and Renilla lucifereses
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-Luciferase [Luciola cruciata] (Japanese firefly)
plasmid size
3770
insert size
1647
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE