Renilla luciferase
Renilla luciferaseBBF10K_003370
source
Renilla reniformis
36kDa protein from sea pansy (Renilla reniformis). It produces a blue light of 480nm, and it requires only coelenterazine and oxygen. It can be used in dual-reporter assays with Firefly luciferase due to their differences in substrate requirements and light output.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-Renilla luciferase
plasmid size
3059
insert size
936
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE