CE_T7RNApol_Stop

CE_T7RNApol_Stop
BBF10K_003441
source
No Value

T7 RNA polymerase defined as a CE part. T7 cannot function with C terminal fusions, so that option is taken away with this part. Has not been successfully synthesized.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … AGGT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-CE_T7RNApol_Stop
plasmid size
4776
insert size
2652
origin
ColE1
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.