CE_T7RNApol_Stop
CE_T7RNApol_StopBBF10K_003441
source
No Value
T7 RNA polymerase defined as a CE part. T7 cannot function with C terminal fusions, so that option is taken away with this part. Has not been successfully synthesized.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … AGGT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-CE_T7RNApol_Stop
plasmid size
4776
insert size
2652
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE