CD_PBCV-1_ligase
CD_PBCV-1_ligaseBBF10K_003442
source
No Value
PBCV ligase defined as a CD part.
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-CD_PBCV-1_ligase
plasmid size
3017
insert size
897
origin
ColE1
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE