Luz-RFP
Fungal Luciferase RFP fusionBBF10K_003484
source
Neonothopanus nambi
catalyzes light reaction of the luciferin. Fused to RFP, which should shift color to red.
attr.
Andreas Stürmer, Sebastian Cocioba, Justin Atkin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 for
reverse primer
M13 rev
Construct
plasmid name
pOpen-Luz-RFP
plasmid size
3653
insert size
1530
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE