Luz-Y233F

Fungal Luciferase Y233F pocket mutation
BBF10K_003486
source
Neonothopanus nambi

catalyzes light reaction of the luciferin. Pocket mutation based off of firefly luciferase color-shifts

attr.
Andreas Stürmer, Sebastian Cocioba, Justin Atkin

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 for
reverse primer
M13 rev

Construct

plasmid name
pOpen-Luz-Y233F
plasmid size
2930
insert size
807
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.