Luz-Y233F
Fungal Luciferase Y233F pocket mutationBBF10K_003486
source
Neonothopanus nambi
catalyzes light reaction of the luciferin. Pocket mutation based off of firefly luciferase color-shifts
attr.
Andreas Stürmer, Sebastian Cocioba, Justin Atkin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 for
reverse primer
M13 rev
Construct
plasmid name
pOpen-Luz-Y233F
plasmid size
2930
insert size
807
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE