H3H

hispidin-3-hydroxylase
BBF10K_003487
source
Neonothopanus nambi

converts hispidin into luciferin. Required as second step in fungal bioluminescence pathway

attr.
Andreas Stürmer, Sebastian Cocioba, Justin Atkin

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 for
reverse primer
M13 rev

Construct

plasmid name
pOpen-H3H
plasmid size
3392
insert size
1269
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

No Value