HispS_1
hispidin synthaseBBF10K_003488
source
Neonothopanus nambi
converts caffeic acid to hispidin. Required as first step in fungal bioluminescence pathway. NOTE: this gene was split because it was too large. Both fragments are required for cloning
attr.
Andreas Stürmer, Sebastian Cocioba, Justin Atkin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 for
reverse primer
M13 rev
Construct
plasmid name
pOpen-HispS_1
plasmid size
5400
insert size
3277
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE