T7_Orf1A_1
SARS-CoV-2 Orf1A positive controlBBF10K_003505
source
SARS-CoV-2
PolT7-driven expression of a 508nt part of the Sars-CoV-2 OrfA RNA, to be used as control
attr.
Open Bioeconomy Lab
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
Escherichia coli
promoter
T7
inducer
LacIq
cloning
method
N/A
enzyme
N/A
overhangs
3' - N/A … N/A - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-T7_Orf1A_1
plasmid size
2665
insert size
664
origin
ColE1
copy number
high (3-500)
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE