T7_Orf1A_2

SARS-CoV-2 Orf1A 2 positive control
BBF10K_003506
source
SARS-CoV-2

PolT7-driven expression of a 269nt part of the Sars-CoV-2 OrfA RNA, to be used as control

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
Escherichia coli
promoter
T7
inducer
LacIq

cloning

method
N/A
enzyme
N/A
overhangs
3' - N/A … N/A - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T7_Orf1A_2
plasmid size
2704
insert size
703
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.