MMSYN1_0544

Heat-inducible transcription repressor HrcA
BBF10K_000262
source
Synthetic bacterium JCVI-Syn3.0

Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.

attr.
Anton Jackson-Smith and Bioe80 class

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-MMSYN1_0544
plasmid size
3080
insert size
1023
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

No Value