OR gate

OR gate
BBF10K_000498
source
No Value

Two-input recombinase logic OR gate. Produces GFP when one or two recombinases have been expressed and acted on the DNA registers (binary output number: 0111). Consists of a promoter followed by one transcriptional terminator flanked by nested parallel recombination sites. Recombination excises the terminator and allows for the expression of downstream GFP. Operated by recombinases Bxb1 and TP901 (e.g. using the dual controller). Low copy pSC101 ori (5-10 copies)- Ampicilin resistance.

attr.
Drew Endy

Usage

growth

shipping strain
DH5alpha
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
N/A
enzyme
N/A
overhangs
3' - No ValueNo Value - 5'

sequencing

forward primer
No Value
reverse primer
No Value

Construct

plasmid name
OR gate
plasmid size
4800
insert size
No Value
origin
pSC101
copy number
5-10

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere

protocols and instructions

No Value

citations

Amplifying genetic logic gates. Bonnet J, Yin P, Ortiz ME, Subsoontorn P, Endy D. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28. 10.1126/science.1232758 PubMed 23539178

intellectual property

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