Dual-recombinase-controller

Dual-recombinase-controller
BBF10K_000501
source
No Value

Contains pTET-Bxb1 integrase inducible by anhydrotetracyclin (AtC) and pBAD-TP901 integrase inducible by arabinose. Can be used to control two-input logic gates. Regulated ColE1 ori (50-75 copies); Chloramphenicol resistance.

attr.
Drew Endy

Usage

growth

shipping strain
DH5alpha
growth conditions
37 C, shaking 300 rpm
antibiotic
Chloramphenicol

expression

strain
No Value
promoter
pTET promoter, pBAD promoter
inducer
Anhydrotetracycline (AtC), Arabinose

cloning

method
N/A
enzyme
N/A
overhangs
3' - No ValueNo Value - 5'

sequencing

forward primer
No Value
reverse primer
No Value

Construct

plasmid name
Dual-recombinase-controller
plasmid size
6614
insert size
No Value
origin
pUC
copy number
50-75

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere

protocols and instructions

No Value

citations

Amplifying genetic logic gates. Bonnet J, Yin P, Ortiz ME, Subsoontorn P, Endy D. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28. 10.1126/science.1232758 PubMed 23539178

intellectual property

We are unaware of third-party property rights claims on uses of this item as of we.