infB

translation initiation factor IF-2
BBF10K_000894
source
Bacillus subtilis 168

Protects formylmethionyl-tRNA from spontaneoushydrolysis and promotes its binding to the 30S ribosomalsubunits during initiation of protein synthesis. Alsoinvolved in the hydrolysis of GTP during the formation ofthe 70S ribosomal complex

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-infB
plasmid size
4174
insert size
2151
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.