secA

protein translocase subunit SecA
BBF10K_001010
source
Bacillus subtilis 168

functions in protein export; can interact withacidic membrane phospholipids and the SecYEG proteincomplex; binds to preproteins; binds to ATP and undergoesa conformational change to promote membrane insertion ofSecA/bound preprotein; ATP hydrolysis appears to driverelease of the preprotein from SecA and deinsertion ofSecA from the membrane; additional proteins SecD/F/YajCaid SecA recycling; exists in an equilibrium betweenmonomers and dimers; may possibly form higher orderoligomers; proteins in this cluster correspond SecA1;SecA2 is not essential and seems to play a role insecretion of a subset of proteins

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-secA
plasmid size
4549
insert size
2526
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.